西村昌彦

西村 昌彦　助手・農博

略歴

  • 1986年　北海道大学水産学部卒業
  • 1992年　東京大学大学院農学系研究科博士課程修了
  • 1992年　東京大学海洋研究所助手
  • 1995-1996年　ブリティッシュカウンシル博士研究員（英国淡水生態研究所）

研究テーマ

わたしの興味は、環境微生物の検出や現存量の評価、及びその“場”を構成する微生物群の種組成の解析です。特に海洋では、顕微鏡的に視認される微生物のほとんどが寒天培地を用いた従来法では分離されないことから、顕微鏡・フローサイトメーターによる細胞レベルでの検出システムと、培養を介在しない遺伝子を用いた分類同定法が必要とされます。遺伝子や光学装置を駆使した分析手法は、実験室内での管理された微生物試料、例えば純粋培養菌株の分析には非常に明瞭な結果を与えます。これらの手法を海水サンプル、海底堆積物などの環境試料に適用して、複雑な群集組成を解析したり、現存量を推定したりすることが、現在の研究の中心課題です。

所属学会

・日本微生物生態学会
・日本水産学会
・日本海洋学会

研究業績

  1. Haider Md. N., Nishimura, M. Ijichi, Ching-chia Yang, W. Iwasaki and K. Kogure. Habitability analyses of aquatic bacteria. J. Oceanogr. 74, 197–207, 2018.
  2. Haider Md. N., Nishimura and K. Kogure. Bacterial community structure and diversity of closely located coastal areas. Open journal of Marine Science, 6, 423-439, 2016.
  3. Saido K., K. Koizumi, H. Sato, N. Ogawa, B. G.. Kwon, S-Y. Chung, T. Kusui, Nishimura, and Y. Kodera. New analytical method for the determination of styrene oligomers formed from polystyrene decomposition and its application at the coastlines of the North-West Pacific Ocean. Science of the total environment, 473-474,490-495, 2014.
  4. Yoshida N., M. Nishimura, K. Inoue, S. Yoshizawa, E. Kamiya, A. Taniguchi, K. Hamasaki and K. Kogure. Analysis of nanoplankton community structure using flow sorting and molecular technique. Microbes Environ., 24, 297-304, 2009.
  5. Wada M., D. Zhang, H-K. Do, M. Nishimura, H. Tsutsumi and K. Kogure. Co-inoculation of Capitella sp.1 with its synergistic bacteria enhances degradation of organic matter in organically enriched sediment below fish farms. Mar. Pollut. Bull., 57, 86-93, 2008.
  6. Nishimura M., T. Shimakita, T. Matsuzaki, Y. Tashiro and K. Kogure. Automatic counting of FISH-labeled microbes by an LED illuminated detecting apparatus. Fisheries Science, 74, 405-410, 2008.
  7. Nomura H., K. Toyoda, M. Yamada, K. Okamoto, M. Wada, M. Nishimura, A. Yoshida, A. Shibata, H. Takada, and K. Ohwada. Mesocosm studies on phytoplankton community succession after inputs of the water-soluble fraction of Bunker A oil. La mer, 45, 105-116, 2007.
  8. Khan N. H., Y. Ishii, N. Kimata-Kino, H. Esaki, T. Nishino, M. Nishimura and K. Kogure. Isolation of Pseudomonas aeruginosa from open ocean and comparison with freshwater, clinical, and animal isolates. Microb. Ecol., 53, 173-186, 2007.
  9. Yoshida A., M. Nishimura and K. Kogure. Bacterial community structure in the Sulu Sea and adjacent areas. Deep Sea Research-Ⅱ
  10. Inoue K., M. Nishimura, B. Nayak and K. Kogure. Separation of marine bacteria according to buoyant density by use of the density-dependent cell sorting method. Appl. Environ. Microbiol., 73, 1049-1053, 2007.
  11. Kamiya E., S. Izumiyama, M. Nishimura, J. Mitchell and K. Kogure. Effects of fixation and storage on flow cytometric analysis of marine bacteria. J. Oceanogr., 63, 101-112, 2007.
  12. Nishimura M., A. Yoshida, K. Toyoda, M. Yamada, H. Nomura, M. Wada, K. Okamoto, A. Shibata, H. Takada and K. Ohwada. Mesocosm experiment on the succession of microbial community in response to oil contamination to coastal seawater. La mer, 44, 59-65, 2006.
  13. Nishimura M., T. Shimakita, E. Kamiya, Y. Tashiro and K. Kogure. Use of an automatic cell-counting system with LED illumination for enumeration of marine bacteria. Fisheries Science, 72, 723-727, 2006.
  14. Yoshida A., H. Nomura, K. Toyoda, T. Nishino, Y. Seo, M. Yamada, M. Nishimura, M. Wada, K. Okamoto. A. Shibata, H. Takada, K. Kogure and K. Ohwada. Microbial responses using denaturing gradient gel electrophoresis to oil and chemical dispersant in enclosed ecosystems, Mar. Pollut. Bull., 52, 89-95, 2006.
  15. Toyoda K., A. Shibata, M. Wada, M. Nishimura, H. Nomura, A. Yoshida, K. Okamoto, M. Yamada, H. Takada, K. Kogure, and K. Ohwada. Trophic interactions among marine microbes in oil-contaminated seawater on a mesocosmic scale, Microbes Environ., 20(2), 104-109, 2005.
  16. Urakawa, H., T. Yoshida, M. Nishimura, and K. Ohwada. Characterization of depth-related changes and site-specific differences of microbial communities in marine sediments using quinone profiles, Fisheries Science, 71,174-182, 2005.
  17. Nayak, B. B., E. Kamiya, T. Nishino, M. Wada, M. Nishimura, and K. Kogure. Separation of active and inactive fractions from starved culture of Vibrio parahaemolyticus by density dependent cell sorting, FEMS Microbial Ecol. Vol.51, 179-186, 2005.
  18. Yamada, M., H. Takada, K. Toyoda, A. Yoshida, A. Shibata, H. Nomura, M. Wada, M. Nishimura, K. Okamoto, and K. Ohwada. Study on the fate of petroleum-derived polycyclic aromatic hydrocarbons (PAHs) and the effect of chemical dispersant using an enclosed ecosystem, mesocosm, The special issue of Marine Pollution Bulletin for EMECS 2001, 47, 105-113, 2003.
  19. Ohwada, K., M. Nishimura, M. Wada, H. Nomura, A. Shibata, K. Okamoto, K. Toyoda, A.Yoshida, H. Takada, and M. Yamada. Study of the effect of water-soluble fractions of heavy-oil on coastal marine organisms using enclosed ecosystem, mesocosms, The special issue of Marine Pollution Bulletin for EMECS 2001, 47, 78-84, 2003.
  20. Urakawa, H., T. Yoshida, M. Nishimura, and K. Ohwada. Characterization of microbial communities in marine surface sediments by terminal-restriction fragment length polymorphism (T-RFLP) analysis and quinone profiling, Mar. Ecol. Prog. Ser. 220, 47-57, 2001.
  21. Urakawa, H., T. Yoshida, M. Nishimura, and K. Ohwada. Characterization of depth-related population variation in microbial communities of a coastal marine sediment using 16S rDNA-based approaches and quinone profiling, Environ. Microbiol. 2(5), 542-554, 2000.
  22. Park Young-Tae, M. Nishimura, and K. Ohwada. Study of marine sulfate-reducing bacterial population using fluorescent in situ hybridization method during decomposing processes of detrital material and polypepton in microcosms, Fisheries Science, 63(1), 105-110, 1997.
  23. Park Young-Tae, M. Nishimura, and K. Ohwada. Detection and enumeration of marine sulfate-reducing bacteria using in situ hybridization with 16S rRNA oligonucleotide probes, Fisheries Science, 63(1), 99-104, 1997.
  24. Park Young-Tae, M. Nishimura, and K. Ohwada. Effects of chemical composition and temperature for the production of volatile fatty acids during anaerobic decomposition process of marine sinking particles, Journal of the Korean Fisheries Society, Vol.29, No. 6, 888-892, 1996.
  25. Nishimura, M., K. Kogure, K. Kita-Tsukamoto, and K. Ohwada. Detection and direct count of specific bacteria in natural seawater using 16S rRNA oligonucleotide probe, Bulletin of Japanese Society of Microbial Ecology, Vol.10, No.3, 109-113, 1995.
  26. Nishimura, M., A. Tsuda, K. Kogure, and K. Ohwada. Application of flow cytometry for measuring changes in intracellular RNA contents of marine bacteria, Bulletin of Japanese Society of Microbial Ecology, Vol.10, No.2, 73-79, 1995.
  27. Nishimura, M., K. Kita-Tsukamoto, K. Kogure, K. Ohwada, and Usio Simidu. A new method to detect viable bacteria in natural seawater using 16SrRNA oligonucleotide probe, Journal of Oceanography, Vol.49, 51-56, 1993.
  28. Nishimura, M., K. Kita-Tsukamoto, K. Kogure, and K. Ohwada. Enumerations of Vibrio cholerae in aquatic environments by MPN-16S rRNA hybridization method, Bulletin of Japanese Society of Microbial Ecology, Vol.7, No.1, 43-46, 1992.
  29. 西村 昌彦・信濃晴雄. ｽﾙﾒｲｶ塩辛の菌相特性に及ぼすﾄﾘﾒﾁﾙｱﾐﾝｵｷｼﾄﾞの影響, Nippon Suisan Gakkaishi.57 (6), 1141-1145, 1991.